Basic Statistics
Measure | Value |
---|---|
Filename | HFKW7BGX9_n01_NCR_061.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7772621 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 22198 | 0.28559220885721814 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 13832 | 0.17795798868875762 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 11365 | 0.14621837344185443 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG | 11190 | 0.14396688066998248 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 11035 | 0.14197270135775306 | No Hit |
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC | 9259 | 0.11912326614149849 | No Hit |
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG | 9215 | 0.11855717653028496 | No Hit |
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG | 9124 | 0.11738640028891155 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC | 9081 | 0.11683317635068018 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 8923 | 0.11480040001950437 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT | 8818 | 0.1134495043563812 | TruSeq Adapter, Index 18 (97% over 40bp) |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA | 8354 | 0.10747983209267506 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCCGC | 1555 | 0.0 | 43.2145 | 33 |
GTCCGCA | 1640 | 0.0 | 41.402077 | 34 |
CGTATGC | 1715 | 0.0 | 38.976246 | 46 |
TCCGCAC | 1730 | 0.0 | 38.842594 | 35 |
GTATGCC | 1800 | 0.0 | 37.719227 | 47 |
CCGCACA | 1780 | 0.0 | 37.358025 | 36 |
TATGCCG | 1815 | 0.0 | 37.214676 | 48 |
CGCACAT | 1835 | 0.0 | 36.238766 | 37 |
GTCACGT | 2205 | 0.0 | 31.586823 | 29 |
AGTCACG | 2205 | 0.0 | 31.586618 | 28 |
ATGCCGT | 2175 | 0.0 | 31.215708 | 49 |
TCACGTC | 2280 | 0.0 | 30.394472 | 30 |
TCGTATG | 2230 | 0.0 | 30.288877 | 45 |
CTCGTAT | 2180 | 0.0 | 29.859821 | 44 |
ACGTCTG | 2375 | 0.0 | 29.470203 | 15 |
GCACACG | 2485 | 0.0 | 28.447527 | 11 |
ACGTCCG | 2475 | 0.0 | 27.433928 | 32 |
CACACGT | 2595 | 0.0 | 27.376343 | 12 |
CTAGCAG | 3270 | 0.0 | 26.220312 | 8 |
GCCGTCT | 2565 | 0.0 | 26.196577 | 51 |