FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7772621
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG221980.28559220885721814No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC138320.17795798868875762No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT113650.14621837344185443No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG111900.14396688066998248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110350.14197270135775306No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC92590.11912326614149849No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG92150.11855717653028496No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG91240.11738640028891155No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC90810.11683317635068018No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG89230.11480040001950437No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT88180.1134495043563812TruSeq Adapter, Index 18 (97% over 40bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA83540.10747983209267506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC15550.043.214533
GTCCGCA16400.041.40207734
CGTATGC17150.038.97624646
TCCGCAC17300.038.84259435
GTATGCC18000.037.71922747
CCGCACA17800.037.35802536
TATGCCG18150.037.21467648
CGCACAT18350.036.23876637
GTCACGT22050.031.58682329
AGTCACG22050.031.58661828
ATGCCGT21750.031.21570849
TCACGTC22800.030.39447230
TCGTATG22300.030.28887745
CTCGTAT21800.029.85982144
ACGTCTG23750.029.47020315
GCACACG24850.028.44752711
ACGTCCG24750.027.43392832
CACACGT25950.027.37634312
CTAGCAG32700.026.2203128
GCCGTCT25650.026.19657751