FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032387
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG188690.2683157226699839No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC139760.19873764057637897No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT108310.15401598347758733No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG105570.1501197246397276No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC88780.1262444743157622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88460.12578943678725302No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC84230.11977440945727248No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG83880.11927671216046558No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA79740.11338966413537821No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG78870.1121525308547439No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG78550.11169749332623476No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC77350.10999110259432537TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14550.038.72492646
CGTATGC14850.038.41448644
GTATGCC15350.036.7069545
GTCACGT16800.032.91571829
TCGTATG18050.031.21638143
AGTCACG18100.030.55160728
ATGCCGT18450.030.53938747
GCACACG20100.028.72745111
ACGTCTG20300.028.44442215
ACGTAGA20550.027.76095432
CTCGTAT19900.027.61168142
CGTAGAG21550.026.79794333
CACACGT21800.026.64776612
CGCGGGG2550.026.0832441
CTAGCAG29800.025.8354268
CACGTCT23100.024.8451214
CTGACTC32100.024.5306072
AGCACAC24550.023.94787410
GCCGTCT24650.023.70976449
CAGTCAC24300.023.0444427
Produced by FastQC (version 0.11.5)