Basic Statistics
Measure | Value |
---|---|
Filename | HFKW7BGX9_n01_NCR_059.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8831827 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 24697 | 0.2796363651597795 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT | 19442 | 0.2201356525665641 | TruSeq Adapter, Index 16 (97% over 39bp) |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 16680 | 0.18886239506276561 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 13208 | 0.14955003081468873 | No Hit |
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG | 11651 | 0.1319206094050529 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC | 11645 | 0.13185267329172096 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 11359 | 0.12861438522289897 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG | 10267 | 0.11625001259648769 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG | 9071 | 0.10270808067232295 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCCGC | 2840 | 0.0 | 52.49891 | 35 |
CCCGTCC | 2935 | 0.0 | 50.801067 | 33 |
GTCCGCA | 2990 | 0.0 | 49.63192 | 36 |
CGTATGC | 3055 | 0.0 | 49.14402 | 46 |
CCGTCCG | 3045 | 0.0 | 48.850388 | 34 |
TATGCCG | 3115 | 0.0 | 47.97273 | 48 |
GTATGCC | 3170 | 0.0 | 47.471592 | 47 |
CTCGTAT | 3460 | 0.0 | 42.98776 | 44 |
GCACACG | 3670 | 0.0 | 42.432526 | 11 |
ATGCCGT | 3555 | 0.0 | 42.132664 | 49 |
ACGTCTG | 3685 | 0.0 | 41.974903 | 15 |
TCGTATG | 3820 | 0.0 | 39.66926 | 45 |
CACACGT | 3950 | 0.0 | 39.070267 | 12 |
CGCATCT | 3770 | 0.0 | 38.712067 | 39 |
CACGTCT | 4180 | 0.0 | 37.004192 | 14 |
AGCACAC | 4300 | 0.0 | 36.866737 | 10 |
TCCGCAT | 4095 | 0.0 | 36.41012 | 37 |
AGTCACC | 4200 | 0.0 | 35.74946 | 28 |
GCCGTCT | 4200 | 0.0 | 35.66249 | 51 |
CATCTCG | 4120 | 0.0 | 35.337868 | 41 |