FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8831827
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT246970.2796363651597795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT194420.2201356525665641TruSeq Adapter, Index 16 (97% over 39bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG166800.18886239506276561No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132080.14955003081468873No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG116510.1319206094050529No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC116450.13185267329172096No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC113590.12861438522289897No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG102670.11625001259648769No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG90710.10270808067232295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC28400.052.4989135
CCCGTCC29350.050.80106733
GTCCGCA29900.049.6319236
CGTATGC30550.049.1440246
CCGTCCG30450.048.85038834
TATGCCG31150.047.9727348
GTATGCC31700.047.47159247
CTCGTAT34600.042.9877644
GCACACG36700.042.43252611
ATGCCGT35550.042.13266449
ACGTCTG36850.041.97490315
TCGTATG38200.039.6692645
CACACGT39500.039.07026712
CGCATCT37700.038.71206739
CACGTCT41800.037.00419214
AGCACAC43000.036.86673710
TCCGCAT40950.036.4101237
AGTCACC42000.035.7494628
GCCGTCT42000.035.6624951
CATCTCG41200.035.33786841