FastQCFastQC Report
Sat 10 Nov 2018
HFKW7BGX9_n01_NCR_058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKW7BGX9_n01_NCR_058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8638823
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG198700.23000818514281401No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT176940.20481956859169356TruSeq Adapter, Index 15 (97% over 40bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC150770.17452608995461535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124820.14448727563928557No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG110310.12769100605487577No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT105810.12248196311002088No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC88490.1024329355978239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28600.046.8652846
TATGCCG28750.046.62076648
GTATGCC29400.045.59003447
ATGCCGT32650.040.836949
TCGTATG33450.040.0706345
CTCGTAT33250.039.89064444
GCACACG36550.038.7767211
ACGTCTG36550.038.39373815
CACACGT38950.036.4772412
AGCACAC40100.035.78021210
GCCGTCT37450.035.13516251
CACGTCT40950.034.78114714
CAGTCAC39750.034.6024327
GTCACAT40450.034.0901529
TCCAGTC45550.030.9640525
AGTCACA44650.030.96184228
GAGCACA47600.030.7307199
CTGAACT47350.030.00661319
ACACGTC48050.029.6417913
TCTCGTA45600.029.54706843
Produced by FastQC (version 0.11.5)