FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16827216
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10034605.963315619173129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG136350.026.52976615
GCACACG140200.025.92546511
TCGGAAG152800.025.608113
CACACGT142450.025.58926612
ACACGTC145450.024.77313
CACGTCT145650.024.7395714
AGCACAC150750.024.48102610
ATCGGAA168350.023.361022
CGTCTGA154600.023.30771316
CTCCAGT143500.023.23292424
CGGAAGA170100.023.1626684
AGGGGGG34950.023.1510891
CTGAACT158200.022.82141519
CAGTCAC128250.022.75070227
CCAGTCA131450.022.43599526
GATCGGA176700.022.2585071
AGAGCAC176500.021.2264148
GAACTCC180650.019.6170821
TCCAGTC163200.019.61441825
TCTGAAC188800.019.49308218
Produced by FastQC (version 0.11.5)