FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5402717
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC521740.9656992953730502TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66620.12330832801347916No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATG62960.11653395874705265TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC75250.055.44003744
TATGCCG75200.055.01148646
GTATGCC76850.054.1491645
CTCGTAT72950.054.02302642
TCGTATG77800.053.57792743
ATGCCGT79400.052.190247
GCCGTCT79050.052.11135549
CTAATCG80600.051.76343533
TCTCGTA77450.050.16113341
AGTCACT86700.048.12148728
ACTAATC87400.047.77656632
TAATCGA87100.047.0963634
GTCACTA89300.046.7600429
TCACTAA90600.046.2049930
ATCTCGT83950.045.90208440
CCGTCTT90650.045.55876550
TGCCGTC92100.045.18351448
CTCCAGT92950.045.036424
CACTAAT93550.044.86062231
CAGTCAC92700.044.8163827
Produced by FastQC (version 0.11.5)