FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4571384
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC354100.774601302362698TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46700.058.8309346
CGTATGC46600.058.50654644
GTATGCC47650.057.5845645
GCCGTCT48000.056.36323549
ATGCCGT49400.055.40353447
CTCGTAT48300.055.3603942
TCGTATG49750.054.6614143
TCTCGTA50750.051.99818841
TGCCGTC52600.051.63374748
CCGTCTT52650.051.58471350
CGACATC53500.049.26050636
ACGACAT54550.049.01807435
CATCTCG54150.048.73329539
GACGACA56800.047.69304734
GTCACGA58100.047.53106329
AGTCACG58050.047.51170728
TCACGAC58450.047.30632830
ATCTCGT55500.047.2325940
CACGACG61050.045.2348131
CTGCTTG60100.044.549257
Produced by FastQC (version 0.11.5)