FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9930275
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT363600.366153001805086TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC55150.052.4822746
TATGCCG55350.052.22992348
GTATGCC57050.050.79625347
ATGCCGT59450.048.80447849
GCCGTCT60750.047.06852351
GTCACGT65850.044.65051329
TGCCGTC65350.044.29092450
TCGTATG65650.044.24823845
CTCGTAT61800.043.9466644
AGTCACG68550.042.94290528
CACGTGA69300.042.57853331
ACGTGAT69750.042.55431732
TCACGTG71000.041.8057530
TCTCGTA66000.040.99096743
GTGATCC78600.037.53853634
CCGTCTT77200.037.22055452
CGTGATC82200.035.98057633
CAGTCAC82900.035.55009527
CCAGTCA83500.035.50423426
ATCTCGT76650.035.25006542
Produced by FastQC (version 0.11.5)