FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9696973
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC351160.3621336266482334TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52600.051.3673246
CGTATGC52400.051.29621544
ATGCCGT54750.049.4153647
GTATGCC56500.047.82161345
TGCCGTC58100.046.5663548
CTCGTAT57850.046.10064742
GCCGTCT58700.046.03050649
TCGTATG61750.043.64222743
TCTCGTA64400.041.0314141
CCTATAC67900.039.99986333
CTATACA70000.038.84886634
ACCTATA71900.037.82382632
CCGTCTT72800.037.30737350
ATCTCGT72650.036.3719640
CATCTCG73050.036.12488639
TACATCT76100.035.2287537
CACCTAT77300.035.18153831
GTCACCT78250.034.57549729
AGTCACC78650.034.53315728
ATACATC78500.034.06252336
Produced by FastQC (version 0.11.5)