FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224473
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC408980.7828157978804752TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80740.15454190307807122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53350.057.33704846
CGTATGC53950.056.89399744
GTATGCC55450.055.29181345
CTCGTAT54850.054.49337442
ATGCCGT57100.053.38810747
GCCGTCT56850.053.37662549
TCGTATG57600.053.1672143
ACCTAGC58950.052.18862532
CACCTAG59500.051.64688531
TCTCGTA57950.050.73225441
TAGCTAT60150.050.2145235
CCTAGCT62100.049.4281833
TGCCGTC62450.048.8704748
AGTCACC64200.048.0839528
GCTATCT61300.048.07396337
TCACCTA64400.047.82592830
GTCACCT65200.047.18542529
CAGTCAC66550.046.4381727
ATCTCGT64000.045.93646640
CTAGCTA66850.045.91520334
Produced by FastQC (version 0.11.5)