FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8533440
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC581920.6819289758878014TruSeq Adapter, Index 7 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162950.19095464431694603No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88420.10361589230134623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC77950.055.45059244
TATGCCG79200.054.75250646
GTATGCC82450.053.400545
CTCGTAT81750.053.13025742
TCGTATG82200.052.24343
ATGCCGT85600.050.45502547
CGGAATA100350.049.07267434
GCCGTCT83450.048.90239349
GGAATAT102200.048.21720535
TCTCGTA92950.047.10459541
GAATATC103400.046.81170336
AATATCT100600.046.02694337
CAGTCAC108600.045.4427927
CTCCAGT109700.045.275624
ACTCCAG111100.044.6420623
AGTCACC111350.044.41583628
CCAGTCA111250.044.39153726
TGCCGTC97100.043.4698948
CTGAACT115250.043.18792719
TCCAGTC116050.042.7062325
Produced by FastQC (version 0.11.5)