FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7885835
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC552890.7011178904960603TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384080.4870505152593226No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314260.3985120155316463No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86830.11010882170372574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC75650.055.88620444
TATGCCG76200.055.39166646
TCGTATG76900.054.70471243
CTCGTAT74300.054.64098742
GTATGCC78400.054.0598345
ATGCCGT82450.051.4053547
TCTCGTA80800.049.98547441
GCCGTCT84900.049.46846449
ACAATCT90700.044.56809637
ATCTCGT91900.043.83380540
TGCCGTC96600.043.6943348
AACAATC94150.043.12082336
CCGTCTT100250.042.13863850
ACCCAAC103950.040.7420932
CTCCAGT106300.040.1716824
CAGTCAC105550.039.99083327
CTGAACT107900.039.8694619
CTGCTTG104550.039.7355157
TGAAAAA108050.039.6147262
CCAGTCA106700.039.55956626
Produced by FastQC (version 0.11.5)