FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12921846
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC651520.5042004060410564TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC93100.053.26907744
TATGCCG93300.052.9298146
GTATGCC99600.050.3197745
GCCGTCT93900.050.17024649
ATGCCGT100250.048.63289647
CTCGTAT102700.047.33566342
TCGTATG107800.045.4207143
TCTCGTA109200.044.3578141
TGCCGTC109500.044.0135748
CCGTCTT116050.041.31827550
TATCTCG118300.041.2116239
ATCTCGT123950.039.3898540
AGTCACC135800.038.8158228
GTCACCA141550.037.3626929
CAGTCAC146650.036.0383627
CCAGTCA151350.034.94235626
CTGCTTG132150.034.774157
CTCCAGT157950.033.88192424
TCCAGTC158850.033.68930425
ACTCCAG164750.032.4408423
Produced by FastQC (version 0.11.5)