FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308028
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG191670.15572762752895916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC119200.029.03996828
ACCATGG126400.027.6070932
TATGCCG16400.027.5299946
GTCACCA127100.027.26250329
CCATGGC131050.026.65411633
TGGCATC94350.026.59696836
ATGGCAT129400.026.47899435
CATGGCA133000.026.10510834
CAGTCAC132700.026.03225127
CCAGTCA137450.025.1327326
CATCGGG32000.024.49925839
CTCCAGT144250.024.1190824
TCCAGTC145150.024.04060425
GTATGCC21450.023.33291245
GCATCGG34850.022.69658538
ATCGCGT19600.021.78505540
CGTATGC18700.020.21345544
AACTCCA174500.020.1187522
CATCGCG33800.019.98460239
CTGAACT183000.019.29950319
Produced by FastQC (version 0.11.5)