FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15623669
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC601410.38493519031925216TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213090.13638921817916136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC105500.049.62855544
TATGCCG105100.049.55103346
GTATGCC111650.046.89487545
ATGCCGT114400.045.5231447
CTCGTAT106150.044.41226242
TCGTATG117850.043.86351843
GCCGTCT117700.043.3252649
CAGGCGA127100.041.08613234
TGCCGTC126500.040.67100548
TCTCGTA120550.038.1780641
AGGCGAT138700.036.5888135
GGCGATC130600.035.29398336
AGTCACC153300.035.20613528
CCGTCTT149200.034.5065850
GCGATCT132200.034.33733437
CACCAGG160850.033.55351631
ACCAGGC163500.032.9668732
CAGTCAC164550.032.79810327
ATCTCGT138400.032.72302640
CTCCAGT165900.032.72286224
Produced by FastQC (version 0.11.5)