FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14014625
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC1214260.866423468341108TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG427560.30508129900015163No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274370.19577405745783422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC169400.057.47776844
TATGCCG171550.056.79821446
GTATGCC172850.056.4520345
CTCGTAT165200.055.99423242
TCGTATG174100.055.74517443
ATGCCGT179950.054.10838347
GCCGTCT183400.052.32681349
TCTCGTA182400.050.25320441
TGCCGTC204000.047.60933348
CTCCAGT214350.046.61873624
CAGTCAC214500.046.17622427
ACTCCAG216600.046.05367723
AGTCACC215550.045.855528
CTGAACT220950.045.52911819
CCAGTCA220650.044.90521226
ATCTCGT204150.044.79640240
CCACTCA222000.044.7276933
CTGCTTG214550.044.06078757
CACTCAA224200.044.0229334
TCTGAAC230650.043.6896418
Produced by FastQC (version 0.11.5)