FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928872
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC111500.11229875861024294TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20350.034.91259846
CGTATGC22400.032.02995744
GTATGCC23000.031.80306645
GCACACG24850.031.26697311
ACGTCTG26100.029.90481815
CTCGTAT24150.029.70894842
TCGTATG24850.028.73123643
CACACGT28300.027.57894912
AGCACAC29450.026.50174510
CACGTCT30100.025.69819314
ACGATAT32050.022.71382335
CAGTCAC33850.022.43699627
GCCGTCT33300.022.28205949
TATCTCG33450.021.65832139
GAGCACA38250.021.1365099
ACACGTC37350.020.70908713
AGTCACC36600.020.5604228
TCTCGTA37550.019.0138841
CCAGTCA40100.018.85267826
TCCAGTC41750.018.69451725
Produced by FastQC (version 0.11.5)