FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10803745
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC211940.19617271603504155TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC34650.046.66512345
TATGCCG35600.045.51815446
CGTATGC36350.044.86784444
GCACACG39700.042.2282111
ACGTCTG42450.039.5775915
CACACGT43150.039.09524512
TCGTATG41850.038.30219743
CACGTCT45900.036.67838314
AGCACAC46950.036.15462510
CTCGTAT42450.035.86453642
GCCGTCT52050.031.33465249
CAGTCAC52250.030.88007227
AACTAAT51650.030.83136635
GAGCACA55250.030.5965569
AGTCACC53900.030.0653328
ACACGTC56500.029.85810913
TCCAGTC55450.029.79189525
CCAGTCA54450.029.56784226
CCAACTA56300.029.21818233
CAACTAA60350.027.54723734
Produced by FastQC (version 0.11.5)