FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9281165
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17800.027.72388646
CGTATGC18750.026.3192144
GTATGCC19450.025.73201245
GCACACG22050.024.60296411
ACGTCTG23700.022.00415215
CACACGT25400.020.94470612
TCGTATG24450.020.61287743
CTCGTAT24050.020.22808342
AGCACAC28500.019.15752610
CACGTCT28400.018.609214
ACACGTC29150.018.01023713
CTAGCAG32050.017.5815668
CAGTCAC33200.015.81329127
GAGCACA36650.015.5658669
AGTCACC34050.015.41895328
GCCGTCT33150.014.88651249
CCAGTCA36700.014.3052126
CTGACTC38550.014.2561672
TCCAGTC38800.014.25245725
GCTGACT37350.014.2464371
Produced by FastQC (version 0.11.5)