FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13378280
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC735440.5497268707188069TruSeq Adapter, Index 22 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT413470.309060656526848No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG147660.11037293284338495No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT147180.11001414232621831No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT142490.1065084599814027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG101600.056.73481446
GTATGCC102850.056.38556745
CGTATGC104150.055.48013744
GGTAGCA111200.053.4449934
CTCGTAT105050.053.43916742
AGTCACG112050.053.2590128
TCGTATG107950.053.2029443
ACGTCTG119100.052.89785815
GCACACG119350.052.5508711
GTAGCAT111200.052.40395435
CACACGT119450.052.38947712
CACGTCT124050.050.7306314
AGCACAC126450.049.82126610
GTCACGG120400.049.65296629
CAGTCAC120900.049.4743527
TCCAGTC128850.047.7256325
GCCGTCT118450.047.60071649
CATCTCG115600.047.56305339
ACACGTC131800.047.56011213
CCAGTCA126150.047.2215126
Produced by FastQC (version 0.11.5)