FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9992739
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT555520.5559236561667427TruSeq Adapter, Index 7 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT407150.4074458464290921No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG147000.14710681425783262No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT100190.10026280081967516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG78700.055.10014348
GTATGCC79050.054.98900647
CGTATGC79100.054.90862746
CTCGTAT78250.054.61055844
ACGTCTG86100.053.09036615
TCGTATG81900.052.9032145
GCACACG87850.052.0304511
AGTCACG84900.051.7820728
ACGAGTG84850.051.56429332
CACGAGT86400.050.6397531
CGAGTGG87000.050.36946533
CACGTCT90550.050.36482614
CACACGT91300.049.9493412
TATCTCG86000.049.6078741
AGCACAC92800.049.2923410
GCCGTCT89700.048.03101351
CAGTCAC91850.047.9391427
GTGGATA92100.047.31093636
ACACGTC97900.046.72520413
AGTGGAT94300.046.50432235
Produced by FastQC (version 0.11.5)