FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9029005
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT857510.9497281261888769TruSeq Adapter, Index 22 (97% over 40bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT194370.21527288998067895No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT119100.13190822244533035No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT112820.12495286025425835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG111250.059.5848348
CGTACGT111850.059.54813834
CGTATGC112200.059.42312246
GTATGCC111850.059.2964947
GTACGTA113100.059.074735
CTCGTAT109000.058.37417644
CCGTACG114750.058.10518633
TACGTAA114900.057.9363436
TCGTATG114100.057.82013345
ACGTCTG125250.055.8326115
GCACACG125800.055.69887511
CACACGT127600.054.83086812
CACGTCT129200.054.0979714
ACCGTAC123500.054.0170632
AGCACAC130050.053.95878610
GCCGTCT122300.053.28637751
CAGTCAC127450.052.78174627
AGTCACC127100.052.7627428
TCCAGTC131150.052.2258525
ACACGTC134850.051.88297313