Basic Statistics
Measure | Value |
---|---|
Filename | HFKVNBGX9_n01_NCR6_022.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9029005 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT | 85751 | 0.9497281261888769 | TruSeq Adapter, Index 22 (97% over 40bp) |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 19437 | 0.21527288998067895 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT | 11910 | 0.13190822244533035 | No Hit |
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT | 11282 | 0.12495286025425835 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 11125 | 0.0 | 59.58483 | 48 |
CGTACGT | 11185 | 0.0 | 59.548138 | 34 |
CGTATGC | 11220 | 0.0 | 59.423122 | 46 |
GTATGCC | 11185 | 0.0 | 59.29649 | 47 |
GTACGTA | 11310 | 0.0 | 59.0747 | 35 |
CTCGTAT | 10900 | 0.0 | 58.374176 | 44 |
CCGTACG | 11475 | 0.0 | 58.105186 | 33 |
TACGTAA | 11490 | 0.0 | 57.93634 | 36 |
TCGTATG | 11410 | 0.0 | 57.820133 | 45 |
ACGTCTG | 12525 | 0.0 | 55.83261 | 15 |
GCACACG | 12580 | 0.0 | 55.698875 | 11 |
CACACGT | 12760 | 0.0 | 54.830868 | 12 |
CACGTCT | 12920 | 0.0 | 54.09797 | 14 |
ACCGTAC | 12350 | 0.0 | 54.01706 | 32 |
AGCACAC | 13005 | 0.0 | 53.958786 | 10 |
GCCGTCT | 12230 | 0.0 | 53.286377 | 51 |
CAGTCAC | 12745 | 0.0 | 52.781746 | 27 |
AGTCACC | 12710 | 0.0 | 52.76274 | 28 |
TCCAGTC | 13115 | 0.0 | 52.22585 | 25 |
ACACGTC | 13485 | 0.0 | 51.882973 | 13 |