FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8173603
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT237630.2907285807739867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT188320.2304002286384597TruSeq Adapter, Index 21 (97% over 40bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG85630.10476408017370063No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT84600.10350392599200132No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT81900.10020060920502256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45100.046.4073211
GTATGCC31400.046.2575847
CGTATGC30650.045.90360346
TATGCCG31150.045.16816748
ACGTCTG45700.044.9580715
CACACGT47100.044.43712
GTCACGT44750.044.11298829
AGTCACG44650.044.05500428
CACGTCT47800.043.12963514
CTCGTAT33000.043.05893744
AGCACAC49100.042.41179710
TCGTATG33350.041.87243745
CGTTTCG47700.040.79706233
CAGTCAC49150.040.3768127
CACGTTT48650.040.36061531
ACACGTC55150.037.69711313
CCAGTCA53250.037.26775426
TCCAGTC54900.037.04022225
GAGCACA56600.036.9155279
GTTTCGG56050.035.0311534
Produced by FastQC (version 0.11.5)