FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7230327
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT236970.32774451280004346TruSeq Adapter, Index 20 (97% over 44bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT74890.10357761135837977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC35450.050.84515847
CGTATGC35700.050.5871446
TATGCCG36200.049.40534648
GGCCTTA37000.049.37696536
GCACACG39450.048.52906811
GTCACGT38500.048.45651229
CTCGTAT37250.047.44863544
ACGTCTG40250.046.95680215
AGTCACG40050.046.6685628
GCCTTAT39050.046.336737
CACACGT41000.046.18256412
TCGTATG39450.045.5123245
TCACGTG40900.045.4419430
CACGTCT43400.043.79088614
AGCACAC43600.043.66907510
TATCTCG42150.042.34783641
CACGTGG43800.042.1135831
CAGTCAC45000.041.53415327
GCCGTCT43750.040.479451
CCAGTCA47000.039.46831526
Produced by FastQC (version 0.11.5)