FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9428389
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT439650.46630447683055926TruSeq Adapter, Index 19 (97% over 40bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT109300.11592648542608922No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT101000.10712328479446488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC66300.052.15479346
TATGCCG66750.051.9088748
GTATGCC67200.051.6133547
ACGTCTG73550.049.8239115
CTCGTAT68350.048.84955644
TCGTATG71150.048.64880445
GCACACG76200.048.22796211
AGTCACG72900.047.86833628
CACACGT77150.047.72432312
GTCACGT73700.047.44397429
TCACGTG75550.046.2356330
CACGTGA75300.046.15721531
CACGTCT79550.045.97749714
AGCACAC80750.045.8133910
ACACGTC82000.044.68819413
ACGTGAA80800.043.40496432
GCCGTCT80400.042.9216351
CAGTCAC82550.042.1023127
GAGCACA89250.041.5680779
TCTCGTA80300.040.79538743
Produced by FastQC (version 0.11.5)