FastQCFastQC Report
Thu 15 Nov 2018
HFKVNBGX9_n01_NCR6_009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12606824
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC374390.29697408324253594TruSeq Adapter, Index 9 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG322230.25559966570485954No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC278400.22083278072256737No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG211000.16736967217119872No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT206600.16387949891265238No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC147690.11715083830788786No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG143750.11402554679909864No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA141440.11219320583836181No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG132370.10499868959858566No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG129540.10275387361638427No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC129010.10233346638296847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59500.049.58609446
GTATGCC59300.049.5176545
CGTATGC60100.049.03321544
TCGTATG65600.045.4021843
CTCGTAT63350.045.19195642
ACGTCTG70700.044.90225615
GCACACG71850.044.37648811
AGTCACG67550.043.8874228
ATGCCGT68450.043.10344747
CACACGT74300.042.72477712
GTCACGA71250.041.55939529
CACGTCT77500.041.0077714
AGCACAC79550.040.2130810
ACACGTC80500.039.52160313
CAGTCAC80150.036.98779727
CTGAACT86150.036.68937719
GCCGTCT80300.036.6118549
TCTCGTA77550.036.55591641
TCCAGTC85150.036.25397525
GAGCACA89500.035.859939
Produced by FastQC (version 0.11.5)