FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15801431
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG433480.2743295844534587No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC301770.19097637422838476No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT224980.14237950980515626No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG207690.13143746284751046No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC187790.11884366675397942No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG179180.11339479316778336No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG171820.10873698717540202No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG166770.10554107409639038No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC160560.10161105029031865No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA160470.10155409342356397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC27300.033.46025545
TATGCCG27800.032.6066646
CGTATGC28400.031.67131844
GCACACG39350.028.46205311
ACGTCTG40500.027.65474515
CTCGTAT32950.025.59857242
CACACGT44250.025.23138812
ATGCCGT35150.024.79286647
CACGTCT46400.024.5156214
TCGTATG34700.024.50914643
AGCACAC47450.023.898310
CTAGCAG64350.023.5503628
CTGACTC66750.022.968392
CAGTCAC50350.022.03580927
GTGCGCA146700.020.6845468
TGCGCAT148200.020.47518269
CCAGTCA56150.019.94662526
GAGCACA57500.019.7822889
CTGAACT58950.019.77223419
TCCAGTC57050.019.69330625
Produced by FastQC (version 0.11.5)