FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15371408
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG427930.2783934952478003No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC268170.17446027065315034No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT236160.153635893341716No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG193940.12616931383253896No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC174010.11320368309786585No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG171470.11155126452957334No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG167940.10925479305474164No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG159920.10403731395328261No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA157860.10269716346088792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27250.031.33815246
CGTATGC28100.030.5147544
GTATGCC28050.030.44427345
CTAGCAG68050.026.3840078
CTGACTC70150.025.2989482
ACGTCTG37900.024.65701515
GCACACG38700.024.23711811
ATGCCGT35400.024.12337547
TCGTATG34950.023.73284543
CTCGTAT31150.023.59452842
GCTGACT78750.022.8489271
GTGCGCA149350.021.58298968
ACACAGT42950.021.43268432
CACAGTG43500.021.00029433
CACACGT44050.020.97556912
TGCGCAT153700.020.92660569
CACACAG44900.020.57995431
CACGTCT45950.020.41344514
TAGCAGA91000.020.0760949
ACTCTAG125650.019.7796215
Produced by FastQC (version 0.11.5)