FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11695873
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG317940.27183947705314515No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC224470.19192239861017643No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT166630.14246905724780012No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG154870.1324142285060722No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG135310.11569038070095322No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC132810.11355287459089201No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG130650.11170606931179912No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA128620.10997041435042941No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG127470.10898716153980126No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC117400.10037728692847468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19250.029.45335445
TATGCCG19400.029.40602746
CGTATGC20800.027.59503744
CTGACTC51900.024.3473452
CTCGTAT23850.024.21306642
CTAGCAG53400.024.1191848
TCGTATG24500.023.57047543
GTCACGC24400.023.0947129
GCACACG24900.023.05147411
AGTCACG24150.023.04402428
CACGCCA25000.022.40043431
ATGCCGT25150.022.26569247
ACGTCTG26500.022.18901415
GCTGACT60000.021.528181
GTGCGCA110600.021.20194668
TGCGCAT113750.020.64558469
CACACGT29050.020.2405812
CCGGGGT8750.019.6028941
TAGCAGA66450.019.5930549
ACGCCAA30050.019.56776832
Produced by FastQC (version 0.11.5)