FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12865850
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG396620.30827345258960737No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC269820.20971797432738606No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG203870.15845824411134904No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT202080.1570669640948713No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC165750.12882942052021437No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG164880.12815321179712186No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG162280.1261323581419028No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA160210.12452344773178609No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG152160.11826657391466557No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC149310.11605140740798316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT60300.024.1492211
CTGACTC59850.024.0954382
CTAGCAG61650.023.672958
TATGCCG18350.021.93392246
GTATGCC19350.021.34300445
CGTATGC19250.021.27206444
GTGCGCA134850.021.12635668
TGCGCAT138900.020.56075369
ACTCTAG80500.019.5667365
GACTCTA79750.019.2240644
AGGTGCG147700.019.19356566
GGTGCGC150800.019.17041467
TAGCAGA79050.018.728149
ACTGACC21800.018.14269332
GCACACG22950.017.84278911
ACGTCTG23850.017.46378515
CTCGTAT23600.017.20298842
CTATCGA84450.016.9511217
CTCTAGC87300.016.5591326
CGCGGGG9850.016.34741
Produced by FastQC (version 0.11.5)