FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9819715
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG272770.2777779192165964No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC222040.2261165420788689No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG166050.16909859400196442No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT134310.1367758636579575No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA119280.12146992046103171No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC116660.11880181858638464No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG114710.11681601757281143No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC112730.11479966577441403No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG106430.10838400096133136No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG102650.10453460207348177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18450.030.54118746
CGTATGC19350.029.8441644
GTATGCC19450.029.51077745
CACTTAG24750.025.31322731
CTCGTAT22650.025.18696442
TAGGCAT23400.024.97758135
TCGTATG23950.024.2582243
CTGACTC43900.024.080852
ACGTCTG25450.024.06725515
GCACACG25450.024.06627511
ATGCCGT25200.022.77728347
TTAGGCA27050.022.77237934
CTAGCAG45250.022.58488
GCTGACT48850.021.9285831
GTGCGCA93450.021.53511268
TCACTTA29400.021.30960330
TGCGCAT95850.020.8499469
CACACGT29900.020.71861312
CACGTCT30200.020.39763514
CATCTCG28300.020.03449239
Produced by FastQC (version 0.11.5)