FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13934817
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG395450.283785571062756No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC254960.1829661631006708No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG205100.1471852841698603No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT204690.14689105712690737No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG174410.1251613135644336No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC170180.12212575163348036No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG157550.11306212345666256No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG150280.10784497564625356No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC146020.10478788490727937No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA144500.10369709196755149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24200.027.7675346
GTATGCC25400.026.18000445
CGTATGC26100.025.4779544
CTAGCAG64050.024.7534168
CTGACTC65850.024.6122532
GCTGACT68950.023.6593481
GTGCGCA139200.021.49709768
TGCGCAT143400.020.98950869
ATGCCGT31650.020.89989947
CTCGTAT32700.020.4426442
GCACACG36250.020.27573811
ACGTCTG37300.020.08066715
TCGTATG32550.019.99923343
AGGTGCG152450.019.69743766
GGTGCGC158700.019.29674567
TAGCAGA84450.018.8569959
GACTCTA95050.018.4505884
ACTCTAG93400.018.32685
CTCTAGC90350.018.2868336
CACACGT40400.018.19295912
Produced by FastQC (version 0.11.5)