FastQCFastQC Report
Wed 14 Nov 2018
HFKVNBGX9_n01_NCR6_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKVNBGX9_n01_NCR6_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14840267
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG428900.28901097264624687No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC342700.2309257643410324No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG253420.1707651216787407No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT225400.15188405976792735No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC204950.13810398424772277No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG185720.12514599636246437No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG170870.11513943785512754No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG169850.11445211868492662No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC168610.11361655420350592No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA164320.11072577063471971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33550.034.21642346
GTATGCC34300.034.1826345
CGTATGC36000.032.5683444
GCACACG41700.028.70483411
ATGCCGT42650.027.08024447
GCTGACT60350.026.914131
ACGTCTG44150.026.87482615
CTCGTAT42800.026.24937442
TCGTATG44600.026.05292743
CACACGT49900.023.98796812
ACACGTC51450.023.19727113
CACGTCT51750.022.79287714
AGCACAC53950.022.44632510
CTAGCAG74600.022.2850678
CTGACTC75350.021.8798872
TGCCGTC54250.021.4833648
GACTCTA80950.021.447284
ACTCTAG81500.021.431395
GAGCACA60300.020.4308289
CAGTCAC57850.020.08628827
Produced by FastQC (version 0.11.5)