FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21511112
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6066612.8202214743710137No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN511530.23779802736371788No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN451830.21004492933698637No Hit
CTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN253260.11773449926716945No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA239400.1112913177152348No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA223740.10401135933837359No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN219050.101831090833426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT40700.056.243441
CTTGTTA52100.050.43551
CACCTGG86150.045.039533
ACCTGGC93950.041.0666354
TCCAATC66850.038.8763122
CCTGGCC111400.035.6353655
TCAAAGG79700.035.4914177
GACATCG133400.035.378613
GCCCGGA107900.035.0901879
CTGGCCC120550.033.1747866
CTCCTGG102000.031.9152036
TCCACTC108450.030.8203892
GCTTGTA80000.030.4781387
CTAGCAT88100.029.9933131
TGGCCCG135850.029.5520277
TCCTGGT111700.029.0827757
GAGCGCT84650.028.3549673
CCTGGTG115150.027.9549928
ACTCCTG121650.027.4866265
GGCTCCA82850.027.4689126