FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n02_T1cD5_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n02_T1cD5_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25412568
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1142570.4496082410876382No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA509240.20038903585029266No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG496740.19547020985836616No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA465830.18330693694553027No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT453650.17851403289899706No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA449840.17701477473665786No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT441170.17360307702865763No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA430360.1693492763108396No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT415460.16348603572846318No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC407630.1604048831271204No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG407100.16019632490506272No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA400410.15756376923418366No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT391330.15399073403364824No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG387530.1524954109321026No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC383180.15078365948691214No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT382160.15038228328597095No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG376440.14813142851206537No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC373800.1470925724625705No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGA369750.1454988728411863No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA369060.14522735364643197No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC367990.14480630214152304No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC367750.14471186068247807No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG355130.13974581396102903No Hit
GTGGTATTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCC350460.1379081405704453No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC349760.13763268631489742No Hit
CAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGG328390.12922346139909985No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG325640.12814131968087603No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT314620.12380488268639359No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA306490.1206056782612446No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT297100.11691065617610938No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG290900.11447091848411385No Hit
CGGGGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCG284140.11181081738767999No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA277230.109091690379343No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT275100.10825352243031873No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA269880.10619942069609022No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG268500.10565638230658153No Hit
CAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTC259960.10229584038889733No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC256060.10076116667941627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTGG249500.082.1576843
GGCGAGC87050.080.147741
ACCTGGC256100.079.4484254
CCTGGCC280000.073.4526445
GGCCGAC48150.072.147991
GTCCAAT107700.067.67611
CTGGCCC306550.067.115586
TCCAATC111000.066.715952
GCCCGGA308650.066.243039
TGGCCCG332950.062.409537
CGTCCAA48800.061.3783531
CAAAGGA148250.058.679178
TCAAAGG153850.058.1935127
CGGGGTC20750.057.3206021
CCTTGGG18500.054.848347145
GGAGTCT74200.054.7355041
GCGAGCG128300.053.4234662
GAGTCTA76000.052.8716742
GCAGGCT146400.050.0924224
GCCGCAG148500.049.6683241
Produced by FastQC (version 0.11.5)