FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n02_T1bD6_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n02_T1bD6_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26328206
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA943180.3582393726332892No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG665680.2528391034315061No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT497320.1888924752411919No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG475730.1806921443868982No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA473760.17994389743076306No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC473120.17970081212521657No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC463060.17587981497865823No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC455290.17292860744100833No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT454790.17273869704605016No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT421210.15998431492065962No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG414310.1573635514702369No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA409370.1554872367680502No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC396460.1505837503702303No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA394750.14993425681947337No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG392820.14920120269493486No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC383610.1457030532198054No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG381190.14478388690820787No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG378350.14370519586484548No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG375490.14261890840568475No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC341770.12981135136970592No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT341060.12954167860886534No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC328910.12492685601138186No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA320620.12177814166297543No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA320020.12155024918902564No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT316550.12023227104801595No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT314490.1194498402207883No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT309720.11763809505288739No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG303360.11522243482901949No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG301530.11452736278347261No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA299680.11382469432212737No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT290220.11023158964951885No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG288620.10962387638565271No Hit
GAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTG287550.10921746814044223No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT276180.1048989057590935No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA276180.1048989057590935No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA266080.10106271578093852No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA265720.10092598029656863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT85600.096.588771
TCCAATC92600.090.943082
CTTGTTA100350.072.851531
TCAAAGG130300.072.165767
GGGTCTG90750.070.976522
CCGGCAT29400.069.072681
CAAAGGA133900.068.436998
GGTCTGG97750.066.6392753
GCGAGCG164500.065.736611
TGGGTCT79250.065.5253451
TTACGAC114700.063.3137865
CGATGGG30650.062.4696431
GTTACGA117650.061.5974044
GCGCCTT20000.059.4715731
ATCAAAG167850.059.388586
CGGGTCT26100.054.186321
CGGGGTC19050.054.0616041
CGGCATC33500.053.481132
GGGCACT13100.053.1490481
ATGGGTC41750.051.2549973
Produced by FastQC (version 0.11.5)