FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21511112
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5093802.3679854393394444No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA374050.17388687297988126No Hit
TGTGAAGTGAGTACGGTTTGATCCATGCAGTGTAACATTAAAATGAAAAC239020.11111466482997252No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT224570.10439720642986751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT39200.068.021651
CTTGTTA53800.056.083591
TCCAATC52800.051.9556472
TCAAAGG73100.045.021757
CACCTGG96750.044.8153
GACATCG169450.044.0954673
CCGGCAT26900.042.5191881
ACCTGGC106700.040.5086444
GAGCGCT78100.036.4721073
CTAGCAT93750.036.0765841
GGCATCG25850.035.011853
GCCCGGA123250.034.9408239
CATCGAA263300.034.032415
CCTGGCC131750.033.713655
GGAGGGG73250.032.9488872
GCTTGTA90800.032.46677
ACATCGA253000.032.4486584
GGCTCCA107100.032.0390856
TCTGGAG57450.031.6346025
GGGGAGG68150.030.9920481
Produced by FastQC (version 0.11.5)