FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n01_T1cD5_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n01_T1cD5_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25412568
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1253910.4934212079629261No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA569250.22400333567233346No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA538810.21202501061679402No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG528900.20812536537039467No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT506500.19931082919286236No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA491710.1934908742792149No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT484120.19050416313691712No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA447000.17589721747129217No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG435710.17145453383538414No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT432290.17010874304399304No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA430970.1695893150192456No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG430290.1693217308852848No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT427030.16803890106659036No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGA421810.16598479933236185No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC421460.16584707220458791No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC417730.16437929452859704No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC415440.1634781656068761No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT414580.16313975037863154No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG387190.15236161886512217No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC367450.14459380885867182No Hit
CAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGG359530.1414772407101872No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC353870.13924999630104284No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT351800.13843543871677982No Hit
GTGGTATTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCC351710.13840002316963795No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT345360.13590125956573929No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG337400.1327689511740805No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA337110.1326548344110678No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA336140.13227313351409428No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA328900.12942414949957046No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG324660.12775568372310897No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG309090.12162879406756531No Hit
GTTAAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTG303720.11951566642143367No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT301680.11871291401955127No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA300800.11836662866971964No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA294920.11605281292311741No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG294900.11604494280153034No Hit
CAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTC291420.11467554164537798No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC284700.1120311807921183No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA284580.11198396006259578No Hit
CTGGCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGG278280.10950487176266484No Hit
GCGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCA273230.10751766606192652No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC267700.10534157744309823No Hit
CGAATAAGTAAAGAATCGATGAAAGTAGTGGTATTTCACTGTTGGTATTT267430.10523533080167263No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC266380.10482214941835079No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG264520.10409022811075214No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT263500.10368885190981092No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG260250.10240995715191004No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA255870.10068640052433898No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT255160.10040701120799757No Hit
GGCTTGGCAAACACAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAG254800.10026534901943007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGC76800.087.629321
CACCTGG242900.083.634653
ACCTGGC242550.083.544954
CCTGGCC278250.073.5552445
GGCCGAC46050.071.969631
GTCCAAT100250.069.7357251
TCCAATC102250.069.419182
GCCCGGA296950.068.359799
CTGGCCC299100.068.306046
CGTCCAA45650.064.9748461
GCGATGG35100.063.63651
TGGCCCG323500.063.085587
CAAAGGA136250.059.7543078
TCAAAGG141250.058.562987
GGGCACT13150.058.4578481
CCTTGGG21300.057.52584145
GCGAGCG118700.056.2560232
GAGTCTA70600.055.5587842
GGAGTCT70350.055.460131
CGATGGG40350.055.3435942
Produced by FastQC (version 0.11.5)