FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n01_C1cD9_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n01_C1cD9_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26662696
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT943740.3539552039298652No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC850370.31893623960607737No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA767330.28779160216956307No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC709240.26600460808614407No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA658480.2469667733525522No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG657120.24645669740224319No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC639160.23972069441139787No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC636280.2386405335754494No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT626640.23502499522178852No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA605280.22701380235517066No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC603620.22639120965111703No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC580910.21787369139264837No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA571920.21450193933876754No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT564080.2115615015075745No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG515190.19322502120565752No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT469050.17591994447973303No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG431450.16181784467707241No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC423950.1590049258334566No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT417070.15642454161424638No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC407420.15280525270212733No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT404410.15167633460622287No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC400650.15026612462595682No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT392370.14716066222260493No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG389790.14619301814040112No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT387890.14548041203335177No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT380230.14260748425440548No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA374110.140312142478015No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA374050.14028963912726605No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA372080.13955077911100963No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG364810.13682412311193137No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG363490.13632904939545498No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG363210.13622403375862666No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT362780.13606275974492602No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG360510.13521138297492497No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT344810.12932300619562254No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG337990.12676512532716122No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT332690.12477732934433937No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA326160.12232821467116455No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT324860.12184064207160447No Hit
CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG322460.12094050804164741No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG318350.11939902851534592No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA318070.11929401287851761No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT314120.11781254228754662No Hit
TGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTT311450.1168111431792194No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG308520.11571222955098014No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG305660.11463956983194798No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA300300.11262927049837722No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG296690.11127531889498347No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC296240.1111065437643665No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC293180.10995887287617125No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT290930.10911499722308651No Hit
TATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTG289670.10864242685735906No Hit
GACTAATCGAACTATCTAGTAGCTGGTTCCCTCTGAAGTTTCCCTCAGGA287640.10788106349035373No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG284780.10680840377132156No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC283420.10629832782101255No Hit
GACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTG279810.1049443762176188No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC279810.1049443762176188No Hit
GTTAAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTG273670.10264153332431199No Hit
CAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTC270040.10128008060400194No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA269900.10122757278558778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT59350.097.380581
TCCAATC60100.096.629132
GCGCCTT28500.087.273831
CTTGTTA127350.081.541521
CAAAGGA88900.071.35578
GTTACGA151900.068.967654
TTACGAC152100.068.638125
GGGCACT12900.068.019021
TGGCTCA28600.064.6419453
ATCAAAG101950.062.080716
TCAAAGG114450.060.1769457
TGCCGCA21900.059.9334451
CCGGCAT25450.059.5515751
GTCGCCT10900.058.545151
CACCTGG265700.057.110763
GCGAGCG35750.055.173131
ACCTGGC275600.054.979714
GGCATCG21900.053.2993623
CGGCATC23800.051.7871482
GGGTCTG23950.051.4628032
Produced by FastQC (version 0.11.5)