FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n01_C1bD9_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n01_C1bD9_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23244463
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA839710.36125162366624686No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT576600.24805907540217215No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG554460.23853422640910224No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC542280.23329426883296894No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC538740.23177132549803367No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC533790.22964178608901398No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG482310.20749457623520923No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC471590.20288272523224132No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA463510.19940662858075062No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG449810.1935127518325547No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT436870.1879458346703901No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC421540.18135071565215338No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA420540.18092050567053325No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT406180.17474269033446804No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC398240.17132682308040414No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG395750.17025560022617No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA390970.16819919651402573No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT383230.16486937125628584No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT383020.16477902716014564No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG382580.16458973476823277No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC381830.16426707728201764No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA373600.1607264491332839No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA366790.15779671915845075No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG363660.1564501619159797No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT362030.1557489196459389No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG354600.15255245948250126No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG353530.1520921348021677No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG351340.15114997494241963No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG347690.1495797085095061No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG344320.14812990087144626No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT342950.14754051319662667No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT342660.14741575230195683No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC321780.13843296788572831No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT304960.13119683599487758No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG300490.12927379737703557No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG289790.12467055057370008No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT287000.12347026472497988No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC284710.12248508386706976No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC280530.1206868061438976No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG274250.11798508745932311No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA272830.11737418928542251No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG272730.1173311682872605No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT271940.11699130240178059No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA267930.11526616037548383No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT267220.11496071128853354No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA265720.11431539631610332No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT265700.11430679211647092No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT256480.11034025608593323No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT253830.10920019963463987No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCA253620.10910985553849964No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG251440.10817199777856774No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA250420.1077331835973152No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT250340.1076987667987856No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC250210.10764283950117495No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC246760.10615861506458549No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA244570.10521645520483738No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG238480.10259647641677072No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA235280.1012198044755863No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT234610.1009315637879008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT61700.095.3212051
TCCAATC66150.090.311842
GCGAGCG57450.080.0300451
CTTGTTA96400.077.5595861
TCAAAGG96100.069.6313867
GCGCCTT23900.068.574771
CAAAGGA98800.065.3803948
GGGTCTG36100.064.066472
GTTACGA118400.063.1926964
TTACGAC120650.062.0739065
GGTCTGG38950.059.93623
TGGGTCT31950.059.2411541
ATCAAAG118350.058.746976
GGGCACT10100.058.159071
CTAGCAT107350.057.8263171
CCGGCAT25950.056.7299651
GAGCGCT83650.055.209563
CGGGGTC10500.053.1808471
GGCATCG26000.049.913783
CGGCATC26650.049.785312
Produced by FastQC (version 0.11.5)