HFKNKBGX9_n01_C1aD9_K11777.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HFKNKBGX9_n01_C1aD9_K11777.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21807682
Sequences flagged as poor quality	0
Sequence length	151
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	66057	0.302907021479862	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT	50887	0.23334437837088784	TruSeq Adapter, Index 2 (97% over 37bp)
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT	47282	0.2168135063598231	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	44462	0.2038822833164937	No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG	43462	0.19929674323020669	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG	42969	0.19703607196766715	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	37317	0.17111859939997293	No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG	36695	0.1682663934663024	No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT	35377	0.1622226516325761	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	32504	0.14904839496467345	No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA	32370	0.148433932593111	No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG	32033	0.14688860558403227	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	31969	0.1465951310185099	No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA	31084	0.14253692804214588	No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT	30921	0.14178948500808108	No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG	30827	0.14135844423997013	No Hit
GCTAAATTGATTGGTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTT	30724	0.14088613361108254	No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA	30511	0.13990941357270342	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	30187	0.13842369858474643	No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC	29955	0.13735985328472783	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	29668	0.13604380327996346	No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC	29611	0.1357824274950451	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	29028	0.13310905762473976	No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA	28428	0.13035773357296754	No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA	28109	0.12889494628544199	No Hit
GTTGAAAGCACGACATCTCGTCCGATCTGTCAAGTTAAGCAACGTTGGGC	27285	0.12511646125434148	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	26244	0.12034291402451669	No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT	25471	0.11679829153781682	No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG	25373	0.11634890860936069	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	25149	0.11532174763003239	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	23964	0.10988788262778226	No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT	23592	0.10818206171568351	No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGC	23498	0.1077510209475725	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	22484	0.10310128330007746	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	22317	0.10233549810566753	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	21956	0.10068011813451792	No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA	21938	0.10059757841296477	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTCCAAT	4760	83.030785	1
TCCAATC	5565	71.916565	2
CTTGTTA	7920	66.10916	1
TTACGAC	8660	59.774014	5
GTTACGA	8740	58.97884	4
GCGAGCG	9790	57.18528	1
GGGTCTG	5875	57.014996	2
TCAAAGG	8340	54.937786	7
TGAAAGC	8630	52.674423	3
GGTCTGG	6370	52.583027	3
TGGGTCT	5195	52.48702	1
CAAAGGA	8310	52.431656	8
ATCAAAG	11040	50.827766	6
GCACGAC	9150	49.599056	8
CACGACA	9245	49.40305	9
GCGCCTT	3260	48.494053	1
GAGCGCT	12180	45.952915	3
ACCTGGC	16690	45.78491	4
AAAGCAC	10410	44.2237	5
CACCTGG	18040	43.72556	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content