FastQCFastQC Report
Sun 18 Nov 2018
HFKNKBGX9_n01_C1aD5_K11777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKNKBGX9_n01_C1aD5_K11777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31591019
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA924550.2926622911404029No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT861280.27263444715094504No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG758390.24006506406140304No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA714130.22605475309295975No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC615730.19490666002258428No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC603230.19094983925653047No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG593450.18785402268916998No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC561140.17762643237307413No Hit
CGCCTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACG558000.17663247899664142No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT538730.17053264410369287No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT517440.16379338697495005No Hit
CTTTCATGGGGTCTCATGAGCGGAAAGTTTGGCACTTTAACTCAACGTTT512080.16209670223046618No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG482710.1527997561585462No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC482040.15258767056548572No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG470040.14878912263007407No Hit
AAATAAAGCAGTTATATTCGGTAAAGCGAATGATTAGAGGAATTGTGGGA469360.14857387158040075No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT468530.14831113868153478No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA465420.1473266816749406No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG462100.1462757500794767No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA454910.14399978677484257No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT428230.13555434853177734No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC426710.1350731991266252No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG425430.1346680206801813No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG413590.13092012005057513No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA411920.13039148879623036No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA406680.1287327895311006No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA402520.1274159595801579No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT400170.1266720772761398No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG384520.12171813767704043No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG382900.12120533370575985No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT378270.11973972729401354No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT374370.11850519921500474No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA371210.11750491492534634No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT367730.11640333602407696No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC357370.11312392297317159No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG356240.11276622637592032No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA355140.11241802614850759No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC351340.11121515263562724No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA345090.10923674225260034No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG339890.10759070481392195No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT337080.10670121150571307No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGC332220.10516279959187134No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT327330.1036148913081911No Hit
CTAATTTAAAAGATAAAAAGCATAATGAAAGTAAAGATTATCTTTGGTAT326970.10350093487012876No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG325740.10311158370674905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT69400.078.270851
CTTGTTA131400.075.172581
TCCAATC73550.074.42492
TTACGAC149550.065.979115
GTTACGA151150.065.185074
TCAAAGG105450.059.469367
ACCTGGC252500.055.9899444
CCGGCAT34700.055.1762471
CAAAGGA108000.054.8430188
CACCTGG261250.054.6978873
ATCAAAG141000.053.3715176
CTAGCAT205750.053.2601431
GGGTCTG55100.051.3176772
GCGCCTT31950.049.7108271
GCGAGCG93950.049.0178951
GGTCTGG59200.048.2519383
ATGCCGG58500.047.840046145
GCCCGGA302500.047.0454869
TGGGTCT47150.046.605661
CCTGGCC307600.046.478845
Produced by FastQC (version 0.11.5)