FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13550007
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8664346.3943435601177185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT3550.022.77831
GTGCGCA22950.021.3522568
CTAGCAG18600.021.2566818
CTGACTC18450.020.6875762
TGCGCAT24700.020.12293269
AGGTGCG26400.018.82616466
GCTGACT21250.018.5302331
ACGTCTG28500.016.45060515
GCACACG28750.016.42864811
TCGGAAG33250.016.1123333
CACACGT29550.015.8654812
TAGCAGA25050.015.643679
CACGTCT31500.015.21631414
ACTCTAG30900.015.0649955
AGCACAC31800.014.96351810
CTCTAGC28750.014.8520016
ACACGTC32750.014.6358113
TCTATCG26000.014.53335716
CTAGCAT25900.014.45742426
CTATCGA27150.014.43414117
Produced by FastQC (version 0.11.5)