FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9727534
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT145010.14907169689666466TruSeq Adapter, Index 14 (97% over 44bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT113140.11630902549402553No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT109690.11276239178398142No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA103900.10681021520973352No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG99370.10215333094697998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24850.038.7318146
TATGCCG24800.038.6673848
GTATGCC25850.037.36829847
TCGTATG28000.034.3746645
CTCGTAT28850.032.87679344
GCACACG32350.031.2656311
TCCGTAT32250.030.9288137
CCGTATC31800.030.59623738
CGTATCT31050.030.54641739
ACGTCTG32700.030.5025615
TTCCGTA33000.029.69545236
GTTCCGT33800.029.20014435
CACACGT35850.028.21319812
TATCTCG34200.027.4269141
CAGTCAC36700.027.17998527
GCCGTCT36200.026.68454451
CACGTCT37600.026.52762814
AGCACAC38650.026.35066810
GTCACAG37900.026.31859629
TCCAGTC42950.023.79536425
Produced by FastQC (version 0.11.5)