FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584111
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT117740.1228491614923909No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT107270.111924830586791No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA99360.10367158727606555No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG96510.10069791553958421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17300.033.38070748
GTATGCC17350.033.28450847
CGTATGC17200.032.96484446
GCACACG22950.026.38253811
TCGTATG22000.026.24978345
ACGTCTG22850.026.1913915
CTCGTAT21900.025.57056644
CACACGT26050.023.7802512
CACGTCT26100.023.33253314
CTAGCAG35550.022.8404058
AGCACAC27850.022.4947410
CTGACTC39150.022.0845532
GTCACAG29050.019.75856629
GAGCACA34250.018.5979029
GCCGTCT31000.018.2897951
CAGTCAC31400.018.280227
ACACGTC33750.017.73272513
ACTCTAG72750.017.375
TCCAGTC34350.017.32172825
TCTCGTA33150.017.31509243
Produced by FastQC (version 0.11.5)