FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5293162
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC106830.2018264319134763TruSeq Adapter, Index 12 (100% over 50bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT90610.17118312267790028No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA61250.11571533234765911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC15950.048.4968645
CGTATGC16100.047.61023344
TATGCCG16950.045.84218646
ACGTCTG18050.044.79030215
GCACACG18550.043.20445311
TCGTATG17850.042.94296643
CTCGTAT17200.042.73353242
CACGTCT20300.039.82510814
CACACGT20300.039.65270212
GTCACCT19400.039.5089729
AGCACAC21250.038.04433410
AGTCACC20700.037.3665928
CAGTCAC22050.034.921127
GAGCACA23650.034.776189
GCCGTCT23850.032.57751549
CCAGTCA24000.031.93762426
TCCAGTC24350.031.91007825
CCTTGTA25500.030.60767433
ACACGTC26500.030.50753613
TGTAATC25100.029.97963136
Produced by FastQC (version 0.11.5)