FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7684095
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT80140.10429334879383974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13550.035.38745544
TATGCCG14400.033.54166846
GCACACG15550.032.18502811
GTATGCC15450.031.03560345
ACGTCTG16500.030.12023415
CACACGT18100.027.844412
TCGTATG17600.027.2443243
AGTCACG18200.026.73042528
AGCACAC19250.025.9988210
CTCGTAT18250.025.89024542
CACGGCT19300.025.56911731
GTCACGG19800.024.7465129
GGCTACA19950.024.56076834
CACGTCT20450.024.13093414
GCCGTCT22550.022.34960749
CAGTCAC22400.021.71875427
GAGCACA23250.021.6764359
CTAGCAG26900.021.3371838
GCTACAT22150.021.17326735
CATCTCG22950.020.89243339
Produced by FastQC (version 0.11.5)