FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8231026
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13800.032.9713746
GTATGCC13950.032.6170445
CGTATGC14600.031.4046444
GCACACG18450.026.93665311
ACGTCTG19450.026.27134515
CTCGTAT18350.025.1776942
TCGTATG18650.024.77253743
CACACGT21900.022.53339612
AGCACAC22500.022.39915310
CACGTCT22800.022.10429214
CACTAGC21650.021.82392131
TAGCTTA22400.021.09295534
CTAGCAG26750.020.5409058
TATCTCG22750.020.46101439
CAGTCAC25700.018.79386327
GAGCACA27750.018.5396169
TCCAGTC26550.018.19217725
AGTCACT26850.018.11904728
GCCGTCT25850.018.00716649
TCACTAG27100.017.82242230
Produced by FastQC (version 0.11.5)