FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7935413
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC104780.13204101664273807TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22300.040.49288644
TATGCCG22950.039.34627546
GTATGCC23050.039.02324745
CTCGTAT27050.033.77068342
ACGTCTG27800.033.74004715
TCGTATG27950.033.05892643
GCACACG29000.032.1021311
AGTCACG30200.030.24805628
CACACGT32150.029.28377212
CACGTCT33250.028.20972414
ACACGTC34300.027.24412313
AGCACAC34850.026.91407610
GCCGTCT35500.025.73198749
CAGTCAC36350.025.22703727
CCAGTCA37450.024.29898826
TCTCGTA37700.023.7663541
GAGCACA41150.023.0485829
TCCAGTC40600.022.75897625
CGATCAG41500.022.1800933
CTCCAGT45600.020.49361224
Produced by FastQC (version 0.11.5)