FastQCFastQC Report
Fri 16 Nov 2018
HFKN2BGX9_n01_NCR6_051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFKN2BGX9_n01_NCR6_051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9646233
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14850.024.98330744
GTATGCC16100.022.60870645
GCACACG19700.021.14170511
TATGCCG16600.021.08456646
CTAGCAG34350.019.9699678
ACGTCTG21800.018.46331215
CTGACTC39350.017.9692082
CGCGGGG4900.017.1477551
CACACGT25250.016.9105512
AGCACAC25150.016.83845110
TCGTATG20900.016.57912843
CTCGTAT21600.016.3659242
CACGTCT26000.015.749763514
CGGGCGC18600.015.24194315
CGCTATG22350.015.18977229
GCTGACT46650.015.1597431
ACTCTAG56600.015.1517565
CGGCAAT26850.014.9949711
GGCGGGC19900.014.42189313
GTGTGCG15050.014.4179398
Produced by FastQC (version 0.11.5)